T AND B CELL QUANTITATION BY FLOW CYTOMETRY

General Information

HLAB/HOL Code: TBSM
UPHSM LIS Test #: 775
Schedule: Monday-Saturday
Testing Time: Approx 4 hours
Testing Lab: UP Health System-Marquette

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 5 mL whole blood
Temperature: Ambient
Tube Type: 1 Lavender Top
Collection Info:
For reference accounts, samples must arrive within 48 hrs of collection. Collect samples Monday thru Friday.

Methods

Flow Cytometry

Clinical Utilities

Determining percentages and absolute counts for markers CD3, CD4, CD8, CD19, CD56/16 and total lymphocyte count with marker CD45. Ratio of Helper cells to Suppressor cells is also reported.

CPT Codes

86359 for total Tcell counts (CD3)
86360 for Helper and Suppressor cell total counts (CD4 and CD8)
86361 for Helper cell only total counts (CD4)
86355 for total B cell counts (CD19)
86357 for total Natural Killer cell counts (CD56/16)

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

T & B SURFACE MARKER

% T-cells (CD3)
0-2 months: 53-84%
3-5 months: 51-77%
6-11 months: 49-76%
12-23 months: 53-75%
2-5 years: 56-75%
6-11 years: 60-76%
12-17 years: 56-84%
>=18 years: 59-85% or as indicated on report

% B-cells (CD19)
0-2 months: 6-32%
3-5 months: 11-41%
6-11 months: 14-37%
12-23 months: 16-35%
2-5 years: 14-33%
6-11 years: 13-27%
12-17 years: 6-23%
>=18 years: 5-30% or as indicated on report

% Natural killer (CD16+CD56)
0-2 months: 4-18%
3-5 months: 3-14%
6-23 months: 3-15%
2-11 years: 4-17%
12-17 years: 3-22%
>=18 years: 5-20% or as indicated on report

% Helper cells (CD4)
0-2 months: 35-64%
3-5 months: 35-56%
6-11 months: 31-56%
12-23 months: 32-51%
2-5 years: 28-47%
6-11 years: 31-47%
12-17 years: 31-52%
>=18 years: 32-60% or as indicated on report

% Suppressor cells (CD8)
0-2 months: 12-28%
3-5 months: 12-23%
6-11 months: 12-24%
12-23 months: 14-30%
2-5 years: 16-30%
6-17 years: 18-35%
>=18 years: 16-40% or as indicated on report

ABSOLUTE COUNTS
CD45 lymph count, flow: 0.60-2.90 thou/uL or as indicated on report

T-cells (CD3)
0-2 months: 2,500-5,500 cells/uL
3-5 months: 2,500-5,600 cells/uL
6-11 months: 1,900-5,900 cells/uL
12-23 months: 2,100-6,200 cells/uL
2-5 years: 1,400-3,700 cells/uL
6-11 years: 1,200-2,600 cells/uL
12-17 years: 1,000-2,200 cells/uL
> or =18 years: 500-2250 cells/uL or as indicated on report

B-cells (CD19)
0-2 months: 300-2,000 cells/uL
3-5 months: 430-3,000 cells/uL
6-11 months: 610-2,600 cells/uL
12-23 months: 720-2,600 cells/uL
2-5 years: 390-1,400 cells/uL
6-11 years: 270-860 cells/uL
12-17 years: 110-570 cells/uL
> or =18 years: 50-500 cells/uL or as indicated on report

Natural killer (CD16+CD56)

0-2 months: 170-1,100 cells/uL
3-5 months: 170-830 cells/uL
6-11 months: 160-950 cells/uL
12-23 months: 180-920 cells/uL
2-5 years: 130-720 cells/uL
6-11 years: 100-480 cells/uL
12-17 years: 70-480 cells/uL
> or =18 years: 50-450 cells/uL or as indicated on report

Helper cells (CD4)
0-2 months: 1,600-4,000 cells/uL
3-5 months: 1,800-4,000 cells/uL
6-11 months: 1,400-4,300 cells/uL
12-23 months: 1,300-3,400 cells/uL
2-5 years: 700-2,200 cells/uL
6-11 years: 650-1,500 cells/uL
12-17 years: 530-1,300 cells/uL
> or =18 years: 280-1300 cells/uL or as indicated on report

Suppressor cells (CD8)
0-2 months: 560-1,700 cells/uL
3-5 months: 590-1,600 cells/uL
6-11 months: 500-1,700 cells/uL
12-23 months: 620-2,000 cells/uL
2-5 years: 490-1,300 cells/uL
6-11 years: 370-1,100 cells/uL
12-17 years: 330-920 cells/uL
> or =18 years: 125-946 cells/uL or as indicated on report

LYMPHOCYTE RATIO

H/S ratio: > or = 1.0 - 4.0