FISH ANALYSIS ALK FOR NON-SMALL CELL LUNG CARCINOMA
General Information
HLAB/HOL Code: FISH
UPHSM LIS Test #: 4764
Schedule: Monday - Friday
Testing Time: 1 - 3 days
Testing Lab: UP Health System-Marquette
UPHSM LIS Test #: 4764
Schedule: Monday - Friday
Testing Time: 1 - 3 days
Testing Lab: UP Health System-Marquette
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume:
Temperature: Ambient
Tube Type: Formalin-fixed, Paraffin-embedded Tumor Tissue (FFPET)
Collection Info:
Specimen must be immediately fixed in 10% neutral buffered formalin. The volume of formalin should be at least 10 times the volume of the specimen. Optimum fixation time is 12-24 hours, not to exceed 48 hours or be less than 6 hours. Tissue should then be placed in a paraffin-embedded tissue block. Maintain specimen at room temperature. Label specimen containers with patient's name and a unique identifying number.
FFPET Limitations or Unacceptable Conditions: Specimen fixed in fixative other than 10% neutral buffered formalin or undergo decalcification.
SPECIMENS CANNOT BE FROZEN.
Please provide:
(1) Pathology Report
(2) H & E of the fixed specimen. Region of tumor marked by a pathologist on the H & E.
Temperature: Ambient
Tube Type: Formalin-fixed, Paraffin-embedded Tumor Tissue (FFPET)
Collection Info:
Specimen must be immediately fixed in 10% neutral buffered formalin. The volume of formalin should be at least 10 times the volume of the specimen. Optimum fixation time is 12-24 hours, not to exceed 48 hours or be less than 6 hours. Tissue should then be placed in a paraffin-embedded tissue block. Maintain specimen at room temperature. Label specimen containers with patient's name and a unique identifying number.
FFPET Limitations or Unacceptable Conditions: Specimen fixed in fixative other than 10% neutral buffered formalin or undergo decalcification.
SPECIMENS CANNOT BE FROZEN.
Please provide:
(1) Pathology Report
(2) H & E of the fixed specimen. Region of tumor marked by a pathologist on the H & E.
Methods
Fluorescent In situ Hybridization (FISH) pretreatment of 5 um cut tissue slide. Processing of tissue and analysis of 60 cells. Probes specific for detection of rearrangement of the ALK gene located on chromosome 2p23.
Clinical Utilities
Detection of EML4/ALK rearrangement associated with Non-small cell lung carcinoma.
CPT Codes
88271x2, 88274x1, 88291x1
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
An interpretative report will be provided.
