General Information

UPHSM LIS Test #: 025997
Testing Time: 2-4 Days
Testing Lab: Mayo

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 1.2 mL of Serum
Temperature: Refrigerate
Tube Type: Serum
Collection Info:
Collection Container/Tube: Red top
Collection Instructions: Centrifuge and remove serum from red blood cells within 2 hours of draw.

Specimen Acceptability

Gross hemolysis: OK
Gross lipemia: OK
Gross icterus: OK
Other: Serum gel or SST tube


Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Clinical Utilities

All applications that require moderately sensitive measurement of estradiol:
-Evaluation of hypogonadism and oligo-amenorrhea in females
-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)
-In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women
-Evaluation of feminization, including gynecomastia, in males
-Diagnosis of estrogen-producing neoplasms in males and, to a lesser degree, females
-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males
-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)
-As an adjunct to clinical assessment, imaging studies and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men
-Monitoring low-dose female hormone replacement therapy in postmenopausal women
-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

CPT Codes


* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.