FISH ANALYSIS MYELOMA PANEL

General Information

HLAB/HOL Code: FISH
UPHSM LIS Test #: 5446
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 3.0 mL of BM or 5.0 mL of Blood
Temperature: Ambient or Refrigerated
Tube Type: Sodium heparin (green top)
Collection Info:
Prefer a minimum of 3.0 mL of bone marrow or 5.0 mL of whole blood. Draw into green-top (sodium heparin) tube (s), invert several times to mix (clotted specimens may not work and can compromise results). Label vial with patient's name and a unique identifying number. Maximum time from collection should not exceed 24 hrs.
SPECIMENS CANNOT BE FROZEN.

Methods

Fluorescence in situ hybridization (FISH) Interphase set-up and analysis up to 200 cells for 1q, 17p-, MYC, IGH, CEN 3/CEN 7; and CEN 9 / CEN11; up to 500 cells for t(11;14) CCND1/IGH fusion on plasma cell enriched sample when possible. Reflex to t(4;14), t(14;16) and t(14;20) as needed.

Clinical Utilities

Detection of abnormalities involving 1q amplification, 17p, chromosome 3, chromosome 7, chromosome 9, chromosome 11, t(4;14) FGFR3/IGH, t(11;14) CCND1/IGH, t(14;16) CMAF/IGH or t(14;20) MAFB/IGH translocation most commonly associated with myeloma, MGUS or plasma cell dyscrasia performed on plasma cell enriched sample when possible. FISH probes for t(4;14), t(14;16), and t(14;20) performed as reflex testing only, unless specifically requested or patient has previous history.

CPT Codes

CALL FOR CPTs

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

An interpretive report will be provided.