Ureaplasma species PCR
General Information
HLAB/HOL Code: URRP
UPHSM LIS Test #: 026575
Schedule:
Testing Time: 3-4 Days
Testing Lab: Mayo
UPHSM LIS Test #: 026575
Schedule:
Testing Time: 3-4 Days
Testing Lab: Mayo
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: Varies
Temperature: Varies
Tube Type: Varies
Collection Info:
SPECIMEN REQUIRED
The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is not likely.
Submit only 1 of the following specimens:
Specimen Type: Swab
Sources: Vaginal, cervix, urethra, urogenital, chest/mediastinal; bronchus or lung (donor swab), or upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Swab in transport media: M4, M4-RT, M5, M6, universal transport media, or ESwab
Specimen Volume: 1 swab
Collection Instructions:
Vaginal:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Urethra or cervical:
1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.
2. Place swab back into swab cylinder.
Wound:
1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pelvic, peritoneal, amniotic, prostatic secretions, semen, reproductive drainage or fluid, pleural/chest, chest tube, pericardial, sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung; or nasal washings (only infants <3 months)
Container/Tube:
Preferred: Sterile container
Acceptable: Specimen in 3 mL of transport media: M4, M4-RT, M5, M6, or universal transport media
Specimen Volume: 1 to 2 mL
Specimen Type: Synovial fluid
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red top (no anticoagulant), or sterile container
Specimen Volume: 1 mL
Collection Instructions: Send specimen in original tube.
Specimen Type: Urine-first void, kidney/bladder stone, or ureter
Container/Tube: Sterile container
Specimen Volume: 10 mL or entire specimen
Collection instructions: Urine first void: Specimen can be collected at any time during the day. The patient should not have urinated for at least 1 hour prior to specimen collection. The first voided portion is the initial 20 to 30 mL of the urine stream obtained without cleaning the external urethra.
Specimen Type: Tissue
Sources: Placenta, products of conception, urogenital, respiratory, bronchus, chest/mediastinal, bone, spine, or joint
Container/Tube: Sterile container
Specimen Volume: 5 mm(3)
Collection Instructions:
1. Collect fresh tissue specimen.
2. Submit fresh tissue only, do not add fluid to tissue
3. Refrigerate or freeze specimen.
SPECIMEN MINIMUM VOLUME
Fluid: 1 mL
Urine-first void: 2 mL
Swab: 1 swab
Tissue: 5 mm(3)
Temperature: Varies
Tube Type: Varies
Collection Info:
SPECIMEN REQUIRED
The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is not likely.
Submit only 1 of the following specimens:
Specimen Type: Swab
Sources: Vaginal, cervix, urethra, urogenital, chest/mediastinal; bronchus or lung (donor swab), or upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Swab in transport media: M4, M4-RT, M5, M6, universal transport media, or ESwab
Specimen Volume: 1 swab
Collection Instructions:
Vaginal:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Urethra or cervical:
1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.
2. Place swab back into swab cylinder.
Wound:
1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pelvic, peritoneal, amniotic, prostatic secretions, semen, reproductive drainage or fluid, pleural/chest, chest tube, pericardial, sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung; or nasal washings (only infants <3 months)
Container/Tube:
Preferred: Sterile container
Acceptable: Specimen in 3 mL of transport media: M4, M4-RT, M5, M6, or universal transport media
Specimen Volume: 1 to 2 mL
Specimen Type: Synovial fluid
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red top (no anticoagulant), or sterile container
Specimen Volume: 1 mL
Collection Instructions: Send specimen in original tube.
Specimen Type: Urine-first void, kidney/bladder stone, or ureter
Container/Tube: Sterile container
Specimen Volume: 10 mL or entire specimen
Collection instructions: Urine first void: Specimen can be collected at any time during the day. The patient should not have urinated for at least 1 hour prior to specimen collection. The first voided portion is the initial 20 to 30 mL of the urine stream obtained without cleaning the external urethra.
Specimen Type: Tissue
Sources: Placenta, products of conception, urogenital, respiratory, bronchus, chest/mediastinal, bone, spine, or joint
Container/Tube: Sterile container
Specimen Volume: 5 mm(3)
Collection Instructions:
1. Collect fresh tissue specimen.
2. Submit fresh tissue only, do not add fluid to tissue
3. Refrigerate or freeze specimen.
SPECIMEN MINIMUM VOLUME
Fluid: 1 mL
Urine-first void: 2 mL
Swab: 1 swab
Tissue: 5 mm(3)
Methods
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Clinical Utilities
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and U parvum from genitourinary, reproductive, bone, spine and joint, and lower respiratory sources
This test is not intended for medicolegal use
This test is not intended for medicolegal use
CPT Codes
87798 x 2
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
