Platelet Antibody Screen, Serum

General Information

UPHSM LIS Test #: 028290
Testing Time: 2-4 Days
Testing Lab: Mayo

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 1.5 mL
Temperature: Frozen
Tube Type: Serum-Red Top Only
Collection Info:
If ordering electronically, answer all prompt questions for timely result reporting:
1. Reason for request is required for result interpretation.
a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.
b. Record only the diagnosis pertaining to this test.
c. Record diagnosis description instead of code.
2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No
3. Indicate if the patient has received a platelet transfusion within 72 hours of collection (Transfused platelets will interfere with assay): Yes or No
4. Record the most recent platelet count, if available. If not available, enter "Not Available." Platelet count conversion: 93 x10(9)/L =93 x10(6)/mL =93 x 10(3)/microliter

Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Specimen Acceptability

Gross hemolysis: Reject


Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)

Clinical Utilities

Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens (HLA) to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia

For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.

This test is not recommended for the diagnosis of immune thrombocytopenia (ITP) or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.

CPT Codes


* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.