FISH ANALYSIS LYMPHOMA PANEL, B CELL
General Information
HLAB/HOL Code: FISH
UPHSM LIS Test #: 1384
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette
UPHSM LIS Test #: 1384
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume:
Temperature: Ambient
Tube Type: See Collection Info
Collection Info:
Bone marrow or whole blood:
Collect at least 3.0 mL bone marrow or 5.0 mL whole blood in sodium heparin (green-top) tube(s), invert several times to mix (clotted specimens can compromise results). Maximum time from collection should not exceed 24 hours.
Fresh Tissue:
Obtain minimum of 1 cm(3) of fresh tissue. Place specimen in a screw-capped, sterile container with sterile Hanks solution or normal saline. Maximum time from collection should not exceed 24 hours; if time is delayed send specimen refrigerated.
Formalin-fixed, paraffin embedded tissue:
Specimen must be immediately fixed in 10% neutral buffered formalin; the volume of formalin should be 10 times the volume of the specimen. Optimum fixation time is 12-24 hours, not to exceed 48 hours or be less than 6 hours. Tissue should then be placed in a paraffin-embedded tissue block. Maintain specimen at room temperature. Label specimen containers with patient's name and a unique identifying number. Specimen fixed in fixative other than 10% neutral buffered formalin or undergo decalcification are not acceptable. Please also include pathology report, if available.
Temperature: Ambient
Tube Type: See Collection Info
Collection Info:
Bone marrow or whole blood:
Collect at least 3.0 mL bone marrow or 5.0 mL whole blood in sodium heparin (green-top) tube(s), invert several times to mix (clotted specimens can compromise results). Maximum time from collection should not exceed 24 hours.
Fresh Tissue:
Obtain minimum of 1 cm(3) of fresh tissue. Place specimen in a screw-capped, sterile container with sterile Hanks solution or normal saline. Maximum time from collection should not exceed 24 hours; if time is delayed send specimen refrigerated.
Formalin-fixed, paraffin embedded tissue:
Specimen must be immediately fixed in 10% neutral buffered formalin; the volume of formalin should be 10 times the volume of the specimen. Optimum fixation time is 12-24 hours, not to exceed 48 hours or be less than 6 hours. Tissue should then be placed in a paraffin-embedded tissue block. Maintain specimen at room temperature. Label specimen containers with patient's name and a unique identifying number. Specimen fixed in fixative other than 10% neutral buffered formalin or undergo decalcification are not acceptable. Please also include pathology report, if available.
Methods
Fluorescence in situ hybridization (FISH) Interphase analysis of cells for BCL6, MYC, IGH and BCL2 gene rearrangement utilizing breakapart DNA probe strategy. Detection of gene rearrangement will be reflexed to correlating dual fusion probe strategies, MYC/IGH, CCND1/IGH and BCL2/IGH fusion, as necessary.
FISH probes may also ordered individually.
FISH probes may also ordered individually.
Clinical Utilities
Detection of abnormalities involving BCL6, MYC, IGH, BCL2, t(8;14), t(11;14), and t(14;18) most commonly associated with B cell lymphoma.
CPT Codes
CALL FOR CPTs
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
An interpretive report will be provided.
